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Effect of the use of vitamin “E” in the diluent on the viability of ram sperm

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Abstract

The sperm membrane is damaged in cryopreservation processes; this damage can be minimized using antioxidants such as vitamin E. The objective of the present study was to evaluate the effect of the addition of vitamin “E” on the viability of ram sperm during preservation processes. Two experiments were carried out; in the first, 32 ejaculates were used, which after evaluation were divided into two aliquots for processing; the first received Triladyl + vitamin “E” (T + E), and the second received only Triladyl (T); these aliquots were cooled and stored at 5 °C for 24 h. The viability (sperm motility, integrity, and membrane permeability) was evaluated at 0 and 24 h after dilution. In the second experiment, the same procedure was performed as experiment 1, except that the samples were also frozen, and the viability was evaluated at zero and 48 h post-freezing. Dependent variables were analyzed using mixed models in a split plot design. In experiment 1, the integrity and permeability of the sperm membrane was better in the group: “T + E” (P <0.05). In experiment 2, the vitamin significantly improved (P <0.05) the sperm viability. It is concluded that the addition of vitamin “E” improves sperm viability.

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Funding

The CONACYT for financial assistance to the graduate studies of the first author.

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All of the listed authors have participated in the development of this research and wrote the document.

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Correspondence to Juan Gabriel Magaña-Monforte.

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All study procedures were conducted according to the Mexican Official Standard NOM-062-ZOO-1999 for the production, care, and use of experimental animals. On the other hand, the work was carried out following the Reproduction and Animal Breeding Animal Welfare Committee.

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All of the authors declare that during the experiment, no animals or people were maltreated or injured.

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The authors declare no competing interests.

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Espina-Ávila, J.A., Magaña-Monforte, J.G., Aké-Villanueva, J.R. et al. Effect of the use of vitamin “E” in the diluent on the viability of ram sperm. Trop Anim Health Prod 53, 330 (2021). https://doi.org/10.1007/s11250-021-02764-6

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